1. The urinary system of Gambusia affinis was found to consist of kidney, ureter and bladder.

2. The digestive tract of Gambusia affinis consists of cavum, oesophagus, intestine and anus.

3. Mature male Gambusia affinis vitellogenin was induced by E 2 through exposure to the waterborne.

4. The oogenesis of Gambusia affinis is divided into 6 phases.

5. Objective To explore the feasibility about Xiphophorus helleri, Poecilia and Gambusia affinis as test organisms for 7 day subchronic toxicity test .

6. Objective To study acute toxicity of domestic detergents produced by four different factories on Xiphophorus heller, Poecilia and Gambusia affinis.