1. However, the evidence regarding an associine concerning the nitrofuran and sulfonamide classes of discrepancy and birth defects is mixed.

2. The antibody which generates specific reaction to the nitrofurans is prepared by using the nitrofuran compound to immunize the New Zealand white rabbits.

3. Furthermore, the regulation of nitrofuran residues in chick feeding was studied.

4. Therefore, the detection of nitrofuran metabolites and malachite green residues has been strengthened in all countries of the world.

5. The invention discloses a nitrofuran compound and a preparation method and application thereof.

6. METHODS The dissolution rate of four brands of marketed nitrofuran tablets were checked according to the USP24th edition.

7. From chromatograms of LC/MS, it was shown that the detection of nitrofuran metabolites reached good effect.

8. Objective:To develop a method for determining the metabolites of nitrofuran antibiotics in aquatic products by HPLC-MS/MS using isotope internal standard dilution technique.