1 Reticulin fibres were mild to moderately increased in 10 cases.

2 Objective To study the relationship between reticulin myelofibrosis of bone marrow and acute leukemia cells with plastic embedding.

3 Objective To determine the distribution of reticulin fibre and megakaryocyte in bone marrow biopsy(Trephine biopsy)of chronic myelogenous leukemia (CML).

4 Reticulin silver stain can highlight slender argyrophilic fibrils radiating from central vessels of papillae.

5 Results:Reticulin myelofiberosis led to pseudohypoplastic leukemia shown in bone marrow smear, which could be corrected by trephine biopsy.

6 Conclusion:The diagnose of acute leukemia with reticulin myelofibrosis should combine the bone marrow aspiration with trephine biopsy.

7 Objective:To explore the value of bone marrow biopsy in diagnosing acute and chronic leukemia with reticulin myelofibrosis.

8 Objective:To explore the value of trephine biopsy in diagnosing acute leukemia with reticulin myelofibrosis.

9 Results Gantaikang could effectively counteract the increase of HA, LN and PCIII level in serum, and attenuate hepatic collagen and reticulin proliferation induced by porcine serum in rats.