1. P53 protein was detected mainly in the cytotrophoblast.

2. Conclusion:The purify and amount of extravillous cytotrophoblast cells derived from improved compound enzyme digestion gradient centrifugation method increase significantly.

3. ObjectiveTo investigate the effect of Chinese drugs for tonifying Shen on the bioactivity, cell proliferation, invasion and differentiation of human cytotrophoblast of early pregnancy.

4. PIBF is also expressed heterogeneously by syncytiotrophoblast and part of the villous cytotrophoblast.

5. Results:ERCC2 gene expression in human choriocarcinoma cell line JAR was evidently lower than in human cytotrophoblast cells.

6. Results HB-EGF expression was localized in the cytoplasm of cytotrophoblast and syncytiotrophoblast, but no expression was present in those with spontaneous abortion.

7. PIBF protein is present in human first trimester and term placenta. Double immunofluorescence labelling localised PIBF to the extra villous cytotrophoblast.

8. Conclusion: MMP-2 and MMP-9 be involved in the invasion of the normal human cytotrophoblast cells, while TIMP-1 and TIMP-2 make this invasion controllable.

9. Conclusion Strong expression of PTEN has close relationship with the formation of trophoblastic cells in human placenta, the cells in cytotrophoblast have the ability to proliferate in placenta villi.