1. Recombinant cDNA were analyzed by differential screening.

2. Recombinant plaques with a white phenotype were isolated.

3. It also made all health authorities provide recombinant alternative for children under 16.

4. Recombinant Oct-11 protein binds specifically to an octamer sequence invitro.

5. Recombinant DNA techniques developed for cloning genes.

6. Rabies Recombinant Nucleoprotein, Horse anti-, FITC Conjugate.

7. Aspect fundamentals: the biggest thing is recombinant human insulin.

8. Purpose To identify recombinant human insulin and porcine insulin.

9. Figure 2 . Restriction pattern of the recombinant plasmid.

10. Technically, I am the daughter of recombinant DNA.

11. Drug use frequency of recombinant human insulin increased rapid.

12. In charge of recombinant protein conjugation and purification.

13. After nearly 40 years of development, recombinant DNA technology has become the foundation of biological research.

14. Conclusion: Recombinant T7 phage vaccine expressing xenogenic VEGF can break immunologic tolerance against self-VEGF and induce the producing of specific anti-VEGF antibody.

15. Objective To obtain the recombinant l6KDa protein and evaluate its value in serodiagnosis of tuberculosis.

16. Objective Construct a recombinant plasmid pET 28 a - EDA - EDB , prepare the fusion EDA - EDB protein.

17. In this method, recombinant DNA technology was used to realize data storage, and the sticking model was used to encode information.

17. is a sentence dictionary, on which you can find good sentences for a large number of words.

18. Using the recombinant protein, several investigators have shown that thrombopoietin influences all aspects of megakaryocyte development, from the hematopoietic stem cell to the mature platelet.

19. Objective To develop human recombinant neutralizing IgG monoclonal antibodies to hepatitis A virus (HAV) by baculovirus expression system.

20. Objective: To demonstrate the hematopoietic enhancement activity of recombinant human macrophage colony stimulating factor ( rhM CSF ).

21. The strategy turned out to be successful, and the resultant recombinant viruses produced reasonably large amounts of HbsAg in infected cells.

22. With SDS-PAGE and TLC, it was proved that S-adenosylmethionine synthetase was 58.3% of the total soluble proteins of recombinant strain.

23. DNA is the most important genetic material. So the method and technique of DNA extracting is the basic method for biochemistry, molecular biology and recombinant DNA technology.

24. The partial correlation coefficients among 7 characters in wheat - rye recombinant lines were studied.

25. Objective To study the feasibility and the characteristics of recombinant baculovirus as mammalian gene transfer vector.

26. Certain APIs of low molecular weight, such as antibiotics, amino acids, vitamins, and carbohydrates, can also be produced by recombinant DNA technology.

27. Nesiritide is a synthetic form of brain natriuretic peptide using recombinant DNA technology.

28. We cloned CaGUA1 gene into the vector pCR4, a expression plasmid vector used in C. albicans. We transformed this recombinant clone into the deletion mutant of CaGUA1.

29. The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.

30. Objective:To synthesize bilin binding protein(BBP) gene sequence, express BBP efficiently in Escherichia coli and purify the recombinant protein.

31. However, the recovery of recombinant proteins hinges largely on the ability to effectively induce cell lysis.

32. Objective To investigate the in vitro efficiency of recombinant adeno-associated virus (rAAV2) vector-mediated gene transfection into the rabbit articular chondrocytes.

33. Biochip technology is a great breakthrough of life science after recombinant DNA technology and polymerase chain reaction.

34. Methods The recombinant protein was extracted by metal chelate Sepharose chromatography from E .

35. Objective To construct recombinant plasmid for expression of chymopapain in E.

36. Mammalian cell culture process development for the production of recombinant proteins.

37. In this paper, the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed.

38. Results: The recombinant plasmid pUC 18 E 6 had been got.

39. Objective To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages.

40. AIM To investigate thrombolytic effect of recombinant staphylokinase in vitro and in vivo.

41. Using recombinant nucleocapsid protein GST-N of porcine reproductive and respiratory syndrome virus (PRRSV)as antigen, an indirect ELISA for the detection of antibodies against PRRSV was developed.

42. The invention further relates to a method for preparing such recombinant poxvirus and the use of such recombinant poxvirus as medicament or vaccine.

43. The invention discloses an abortus Brucella vaccine strain S19 marked recombinant strain and an application thereof.

44. Tonghua Dongbao main products are second-generation recombinant human insulin, are insulin giant Novo Nordisk and Eli Lilly, the third to the second generation of insulin-production company.

45. Conclusion The recombinant nucleocapsid protein could be used as an antigen for SARS diagnosis.

46. The recombinant plasmid pPGVT 3 derived from the vector pUC 4 was unstable in the E.

47. The in vitro estrogenic activities of 11 protopanaxadiol type saponins, 4 protopanaxatriol type saponins, salidroside, rutin and isoquercitrin were tested by a recombinant yeast.

47. try its best to collect and build good sentences.

48. Human chitinase, its recombinant production, its use for decomposing chitin, and its use in therapy or prophylaxis against infection diseases.

49. Recombinant AAV vector carrying PDX-1 can not only transduce PDX-1 gene into C2C12 cells, but also increase insulin excretion of insulin-secreting cells differentiated from C2C12 cells.

50. Objective To prepare a novel recombinant immunotoxin with specific killing activity to human hepatocarcinoma.

51. It is the Geneva , Switzerland - based Biotech Company's second recombinant infertility drug.

52. We presented the history of recombinant DNA research and our guidelines, and invited the committee's opinions.

53. In order to study the treatment result of recombinant lysostaphin on experimental mastitis in mice induced by Staphylococcus aureus.

54. The purified recombinant inulinase also had high exoinulinase activity. Therefore, the recombinant inulinase may have highly potential applications in food and pharmaceutical industries.

55. Objective To assess the bio-safety of the recombinant fowlpox virus in pregnant sows and newborn piglets.

56. The positive recombinant clones was sequenced and analysed. The results suggested that FnBPA D region gene was cloned successfully.

57. The results indicated that natural and recombinant Aer toxins had similar antigenicity.

58. This invention relates to a solution preparation of recombinant human erythropoietin.

59. Recombinant human insulin injection Novolin N and oral hypoglycemia agent combination therapy on elderly diabetes mellitus is safety and effective.

60. Food ingredients such as recombinant rennet ( ehymosin ) have also been launched in the marketplace.

61. Field trials of recombinant subunit vaccine of infectious bursal disease (IBD) were made in 9 flocks of commercial broiler chicken with 3 batches of experimental production.

62. Results: Show the dose, methods and effects of recombinant human erythropoietin to premature infants with anemia.

63. The strains with recombinant plasmid of hEC - SOD were induced and expressed as inclusion body.

64. The secretion of recombinant protein to periplasm or extracellular medium has more advantages than the expression intracellular as inclusion bodies.

65. However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

66. Objective To investigate the amelogenin expression of recombinant plasmids PsecTaq 2 A - AMG in COS 1 - cell line.

67. Results: The recombinant L asparaginase had the same quality as that of native products.

68. In the last few decades, with the development of recombinant DNA technology, metabolic engineering has made tremendous advances.

69. Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.

70. The invention discloses brucella abortus immune marking recombinant strain and the application thereof in preparation of vaccine.

71. The safety of intramuscular injection of recombinant adeno - associated virus ( AAV ) - mF to rescue hemophilia B was assessed.

72. Results:The constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.

73. The purified recombinant major outer-membrane protein (MOMP) of Chlamydia psittaci(Cps)expressed in E. coli was mixed with oil adjuvant and manufactured as MOMP-vaccine.

74. Purpose : To give an appraisal of recombinant L - asparaginase.

75. AIM To investigate thrombolytic effect of recombinant staphylokinase in vivo.

76. BACKGROUND: Recombinant human erythropoietin is commonly used for treatment of anaemia.

77. After the extensive news coverage of this momentous event(), the study of genetic engineering and recombinant DNA was thrown into the public spotlight.

78. Biotechnologies for animal nutrition and production are often based on the use of micro-organisms, including those produced through recombinant DNA technology.

79. PURPOSE : To establish a stable cell strain secreted recombinant tissue - type plasminogen activator ( rt - PA ).

80. Objective To detect anti - M 2 autoantibody using recombinant BCOADC - E 2 - OGDC - E 2 ( BO ).

81. The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies.

82. Results The recombinant plasmid with mutant gene was constructed correctly.

83. The optional high cell-density fermentation conditions, purification methods and biological activity of the recombinant enterokinase in E.

84. Objective To investigate the amelogenin expression of recombinant plasmid PcDNA 3 - AMG in COS - 1 cell line.

85. Recombinant tissue-type plasminogen activator (r-TPA) was also used when clinically indicated.

86. A semi - quantitative PCR was developed and used to demonstrate the transcriptase activity of the recombinant protein.

87. Optogenetics combines recombinant DNA technology and optic technology, and is very powerful for cell biology research.

88. Conclusion The AK gene of Blattella germanica has been cloned and the recombinant AK protein has been confirmed with immune activity.

89. Conclusion This ELISA using recombinant OspC was a good method for early diagnostic of Lyme disease.

90. Conclusion: The application of recombinant L - asparaginase was promising.

91. Objective To evaluate clinical effect and safety of recombinant human epithelial growth factor (rhEGF) eye drops for corneal epithelial punctate defect (CEPD).

92. The recombinant serine proteinase of the present invention may be enriched in insect body wall to raise insect body wall degrading speed and raise pesticidal fungal virulence.

93. Objective: To establish a assay for plasmin inhibition with the recombinant TFPI-2.

94. The expression cassette including GFP gene controlled by FPV early and late promoter was cloned into F30 gene in fowlpox virus to gain the recombinant fowlpox virus expressing GFP.

95. AIM : To construct a human recombinant immunoglobulin library containing D - Dimer by using phage surface display technology.

96. Results The absorbed recombinant HO-1 was mainly located in the cytoplasm and cytomembrane of ileal tissue.

97. It was evident that the recombinant streptokinase heberkinasa is an effective and safe thrombolytic agent for AMI.

98. This process of combining inter-species genes, which is called recombinant DNA technology, does not have the checks and balances that are imposed by nature in traditional breeding.

99. Luger and her colleagues next packaged the modified histones they received from Shokat's group into recombinant nucleosomes and chromatin.

100. The reactivity of these recombinant proteins with sera from patients with amebiasis was examined by means of enzyme-linked immunoadsorbent assay (ELISA).

101. Etanercept is a recombinant soluble TNF-alpha receptor fusion protein that binds to TNF-alpha.

102. Fowlpox virus as a vector, delivering recombinant fowlpox virus as vaccines. was summarized.

103. Methods Add the stabilizers prepared with various formula into recombinant MVA virus vector vaccine, then lyophilize the vaccine under appropriate condition.

104. Results:All of C-terminal of the 8 recombinant protein drugs successfully detected as the base peak.

105. Carboxypeptidase B is a necessary enzyme especially in the processing of recombinant insulin and is often widely used in the sequencing of proteins.

106. Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.

107. Objective To obtain recombinant poly-epitope peptides based on selected linear B cell epitope and evaluate its probability as epitope vaccines.

107. try its best to collect and make good sentences.

108. Objective To generate recombinant Plasmodium yoelii BY265 strain which can express green fluorescent protein (GFP) in erythrocytic and mosquito stages.

109. Objective : To find a rapid method of screening positive recombinant plasmid.

110. The conditions of purification, denaturation and renaturation of recombinant Bovine growth hormone(BGH) were researched in order to get a good renaturation result.

111. For instance, you can assume they know what a cell is, but not recombinant DNA.

112. The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.

113. The biological activity of recombinant C 1 - INH was assayed by using the inhibition of C 1 estrolytic activity.

114. The recombinant protein should be useful for type-specific serodiagnosis of HSV-2.

115. The recombinant CYP 3 A 4 plasmid was successfully cloned and effectively expressed in Rosetta ( DE 3 ) 2 pLysS.

116. The invention relates to recombinant polypeptides, in particular antibodies or antibody fragments, which are able to bind the ED-B isoform of fibronectin and block the function thereof.

117. PurposeThe chymopapain gene was amplified, and the recombinant vector was constructed.

118. Three pullulanase positive recombinant phages were obtained from this library using the amylopectin and pullulan plate.

119. Objective To definite the optimal expression condition and purified methods of the recombinant of rat kidney biliverdin reductase in E. coli.

120. The uptake of recombinant somatotropin has been affected by low public acceptance, the lack of adequate and good quality feed and the low genetic potential of animals in developing countries.

121. Results The structures of recombinant plasmids were comfinaed by PCR and sequencing.

122. ObjectiveTo observe the effect of 7 flavonoids on recombinant human protein kinase CK2 holoenzyme activity and investigate their structure-activity relationship.

123. CONCLUSION The jel method of bacterial endotoxin test can be used to pyrogen test the endotoxin in recombinant human erythropoietin injection instead of rabbit.

124. Process control of recombinant Escherichia fed - batch culture should be optimized.

125. This cut DNA is then mixed with the modified plasmid DNA, the two DNA strands join at their sticky ends and are sealed together by the enzyme, DNA ligase, to form a recombinant plasmid.

126. Recombinant activated factor VII in chronic liver diseases: Should we be afraid of thromboembolic events?

127. Methods We construct the rat antisense TIMP 1 recombinant plasmid which can be expressed in eucaryotic cells by RT Nest PCR and the technique of recombinant DNA.

128. The restriction map of the recombinant was analysed and compared with that of human SRY gene.

129. Objective To study primarily the safety of transfering recombinant human proinsulin gene plasmid(RHPGP)into diabetic rats induced by streptozotocin.

130. Some success in interferon production by bacteria has recently been achieved by the recombinant DNA technique.

131. The expression level of recombinant Prn ( rPrn ) is 46.4 % of total cell protein.

132. Methods: The recombinant fusion protein MBP hEra was expressed in the E.

133. Objective To identify the recombinant aldolase (ALD) of Plasmodium falciparum, and to develop monoclonal antibodies (McAbs) against the recombinant ALD.

134. A recombinant HGP44 inhibited HIV-1 replication at a 50% effective concentration of approximately 2.5 micromolar, the researchers note, somewhat less potently than did P. gingivalis extracts.

135. Intravenous administration of recombinant tissue plasminogen activator remains the most beneficial proven intervention for emergency treatment of stroke.

136. Finally a recombinant plasmid named pcNcSRS2, which was identified by PCR, restriction enzyme analysis and sequencing, was obtained.

137. Aim To obtain recombinant hepatitis delta virus antigen and investigate its application to toantibody to HDV.

138. With the development of vaccines, such as recombinant DNA vaccines and synthetic peptide vaccines, the studies on adjuvant become more interested.

139. After purification and refoldation of the recombinant OvPrPC, conformational changes of ovine prion protein was triggered by thermal denaturation.

140. Conclusion The constructed recombinant fowlpox virus showed good biosafety to pregnant guinea pigs and their offspring.

141. The conference led to guidelines for recombinant DNA research, enabling experimentation to continue.

142. Aim a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

143. The technology is often called "modern biotechnology" or "gene technology", sometimes also "recombinant DNA technology" or "genetic engineering".

144. The one third of the course will focus on the principles of recombinant DNA technology. The two third of the course will offer an opportunity for practice in laboratory.

145. Objective: To investigate the effects of recombinant AZURIN protein of P. aeruginosa on growth and apoptosis of U2OS cells.

146. It's the academic guidance that the use of recombinant human growth hormone for anti - aging treatment.

147. Recombinant human - like collagen withattacked to the carboxyl be easily purified by Metal Chelated Affinity Chromatography ( MCAC ) .

148. The drug is a recombinant protein, called Factor VIII , which is necessary to help blood clot.

149. AIM:To evaluate the effect of recombinant hirudin (r hirudin) in reducing neointimal proliferation after balloon angioplasty in rabbits.

150. Objective To heterogeneously express recombinant Mistletoe lectin A chain and B chain gene in yeast, and lay the foundation of further research and large scale production.

151. Objective To observe the effect of recombinant tissue plasminogen activator in the treatment of acute myocardial infarction.

152. The renaturation of gene recombinant protein is the most important technique in the field of biotechnology which's many methods have been established.

153. To develop recombinant human pancreatic kallikrein and lay a foundation of hypertension.

154. Recombinant human insulin gene to expand production capacity 15 times.

155. Conclusion: The recombinant human insulin gene could regulate the insulin secretion with the increase of glucose concentration in vitro.

156. The recombinant KDR - CD was identified to have kinase activity catalyzing theATP in the enzymatic reaction.

157. Conclusion Domestic recombinant streptokinase is an effective, reliable and safe thrombolytic agent.

158. The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.

159. The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.

160. Cell density cultivation and high level expression of recombinant urease A fusion protein in Helicobacter pylori.

161. It was showed from our investigation and analysis that the plants expression system will play an important role in large scale production of recombinant protein in the future.

162. Recombinant DNA is also an excellent example of this problem.

163. Objective To construct recombinant cosmid vector pAxCAwt MC148 from the MC148 gene of molluscum contangiosum virus(MCV).

164. Moreover, separately added KGM and KSAP could also reduce the hardness, gumminess and chewiness of recombinant poultry hams.

165. Insert and ligate any DNA fragment into a vector such as plasmid or viral vector, to form a recombinant DNA.

166. Conclusions The recombinant plasmid constructed is useful for establishing a transgenic mouse.

167. Mammalian cells are the ideal host cells for expressing recombinant protein products.

167. try its best to gather and build good sentences.

168. The use of the medium can reduce the costs for recombinant chymosin production by chymosin recombinant strain chy1 at large scale.

169. AIM To study the effects and kinetics of sodium quercetin 7 sulphate (SQMS) on recombinant human protein kinase CK2 holoenzyme.

170. The time of recombinant peptides medline remain in vivo effect the dosage and therapeutic effect extremely.

171. Objective To developed a recombinant protein vaccine with epitopes on VP1 protein for prevention of foot-and-mouth disease virus(FMDV) spreading by inactive-FMD vaccine.

172. Then the recombinant plasmid was transfected into NK-92 cells with Hpofectamine encapsuled.

173. By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.

174. Coatd plate bar using recombinant GM protein (AFMP1) and employed indirect ELISA to detect antibody tilter of mice serum.

175. Conclusion The immune response level induced by recombinant vaccine is lower than that induced by purified vaccine.

176. The invention discloses a brucella melitensis immune labeled recombinant strain and the application thereof in the preparation of vaccine.

177. Other biochemical characteristics of the purified recombinant inulinase were the same as those of the native inulinase produced by the marine derived P. guilliermondii strain 1.

178. Objective To clone human ornithine decarboxylase antizyme ( OAZ ) mutation gene and express its recombinant protein.

179. On the other hand, the essence of effect of lactate on cell growth was the osmolality action, and the growth rate of recombinant CHO cells decreased with the increase of osmolality.

180. AIM: To observe the inhibitory effect of luteolin on recombinant human protein kinase CK2 holoenzyme and to confirm the inhibitory type by its enzyme kinetic analysis in vitro.

181. Chick chorioallantoic membrane (CAM) assay was performed to determine the activity of the recombinant protein.

182. The screening of human tissue plasminogen activator recombinant plasmid showed that the two methods can recognize the positive colones quickly and correctly.

183. It turns that most of the recombinant protein were inclusion body.

184. The result showed that the recombinant salmon GH has biology activity, it can be sorbed through intestines by fishes and that it has significant growth enhancement.

185. This review tries to have a brief introduction on the progresses of Trichoderma spp molecule biology, such as gene cloning, tansforma-tion system, expression system and recombinant protein expression.