1. Choose method of TCA by comparing with deproteinization method.

2. The deproteinization rate was up to 85%.

3. Deproteinization of shrimp and crab shell powder(SCSP), deacetylation of chitin and depolymerization of chitosan in enzyme method were introduced completely in the article.

4. Deproteinization, decoloration, desalination, and initial classification of glycosaminoglycan from Endothlium Comeum were investigated.

5. ObjectiveTo investigate the optimum conditions of extraction and deproteinization from polysaccharide of Dendrobium devonianum.

6. Crude polysaccharide was gained by hot water extraction, alcohol precipitation, decolorization and deproteinization.

7. METHODS: Bio-derived bone scaffold was prepared with a serial of physical and chemical processes, including degreasing , deproteinization, partial decalcification and freeze drying.

8. Experimental Methodsare: Crude polysaccharide was gained throngh hot water extraction, alcohol precipitation, decolorization and deproteinization.

9. Crude polysaccharides were prepared from powered Spirulina platensis by hot water extraction, alcohol precipitation, deproteinization.