1. To mutarotation of N - acetylglucosamine in water was studied specific rotation.
2. To mutarotation of N-acetylglucosamine in water was studied by HPLC/MS/MS with specific rotation.
3. METHODS Combined HPLC/MS/MS with specific rotation to inspect the mutarotation of N-Acetylglucosamine in water.
4. The result showed that it was linear relationship between concentration and mutarotation.
5. The study has provided available and reliable method for the mutarotation of water.