1, Septocutaneous perforator:In 4 saphena arteria flaps and 6 internal low leg flaps, the colour signals of the axial artery were founded through the intermuscular septum.

2, Method : We accept indwelling needle at vena saphena magna near medial malleolus and cephanus vena at wrist.

3, Results The choice vena saphena magna or the vein puncture success ratio are high, also can supplement the blood volume rapidly therefore wins precious time.

4, Method: We accept indwelling needle at vena saphena magna near medial malleolus and vena at wrist.

5, High concentration emulsified isoflurane could damage endothelial cells and weak the relaxation response of the saphena vessel significantly.