1. Repeating these steps produced a plasmid containing two protein A domains, and then by another step to one with three domains.

2. Plasmid: circular DNA molecules able to replicate independently of the chromosome in microorganisms.

3. However, the genes brought in by a plasmid do not usually become a permanent part of the bacterium's own chromosome.

4. However, as described above, this plasmid was found to exhibit wild type R-M.

5. Chickens were immunized intramuscularly with the naked DNA plasmid.

6. BVTech Plasmid is DNA plasmid drawing software free software.

7. Figure 2 . Restriction pattern of the recombinant plasmid.

7. try its best to collect and make good sentences.

8. Objective Construct a recombinant plasmid pET 28 a - EDA - EDB , prepare the fusion EDA - EDB protein.

9. Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.

10. What will happen to the mobility of the plasmid as you add increasing amounts of ethidium bromide to the gel during electrophoresis?

11. The preparation process of the immunotoxin plasmid includes the construction of the recombinant plasmid, the preparation of the recombinant transinfected engineering bacterium and other steps.

12. The recombinant bait plasmid didn't have self-activating effect and did not show toxicity to yeast AH109 cell.

13. Objective To construct recombinant plasmid for expression of chymopapain in E.

14. Objective: Study on extraction of plasmid DNA with phenol - chloroform from clone.

15. Results: The recombinant plasmid pUC 18 E 6 had been got.

16. Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI.

17. Curing the R plasmids from E. coli J53, the activity of L-asparaginase increased and was close to that of the plasmid free strain.

18. The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.

19. This provides strong evidence that the hybridization signals obtained are highly specific and due to the transfected plasmid.

20. The predicted 5' and 3' boundaries of the expression regions were confirmed by direct plasmid DNA sequencing.

21. We therefore took this data into consideration when calculating the superhelical densities of the different plasmid preparations.

22. T3 symbolizes the T3 RNA polymerase promoter of a bluescript plasmid.

23. The sequences at the 5' and 3' ends of the insertion were confirmed by direct plasmid sequencing.

24. After extracting with phenol-chloroform, it is not necessary to recover the plasmid by ethanol precipitation.

25. PC - 3 cells were transfected with mGM - CSF expressing plasmid or the control vector by lipo - some transfection.

26. Objective To select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration(ALR) for ALR gene therapy.

27. We cloned CaGUA1 gene into the vector pCR4, a expression plasmid vector used in C. albicans. We transformed this recombinant clone into the deletion mutant of CaGUA1.

28. The ability of amphiphilic block copolymers that consist of polyethylenimine (PEI) and poly(L-lactide) (PLLA) to modulate the delivery of plasmid DNA was evaluated.

29. The constitutive expression vector of the present invention is inducible plasmid vector with regulating gene eliminated or deleted.

30. In this review, we summarize the current progresses on plasmid biology in the rare actinomycetes.

31. Methods The adhesion forces of MHCC97H cells with high metastatic potential transfected with sense or antisense KAI1 expression plasmid were measured by means of micropipette aspiration technique.

32. This indicates that S. griseus plasmid (s) codes streptidine phosphatase gene as well as some other involved in streptidine biosynthesis.

33. The recombinant plasmid pPGVT 3 derived from the vector pUC 4 was unstable in the E.

34. Methods: The gastric carcinoma cells had been successfully transfected with plasmid containing the C' distal end antisense sequence of glutaminase gene and called SGC-7901GA in previous researches.

35. AIM: To study the gene silencing effect of short hairpin plasmid vector on aquaporin-4 (AQP-4) in astrocytes cultured in vitro.

36. Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.

37. The strains with recombinant plasmid of hEC - SOD were induced and expressed as inclusion body.

38. Agrobacterium method was used to transform the plasmid into Gerbera hybrida.

39. In 1973, the construction of the first biologically functional bacterial plasmid signaled the beginning of molecular cloning and recombinant DNA technology.

40. Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.

41. Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.

42. Results:The constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.

43. Church recalls seeing plasmid and gene sequences for the first time—penicillinase and insulin, the lac repressor and interferon.

44. We established the techniques of screening the plasmid expression library and cloning the immunogen genes.

45. Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F' plasmid and that from the autonomous F plasmid.

46. The recombinant plasmid pcDNA3 F was stable within the host stain ZJ111 in vitro and in vivo as shown by restriction enzyme analysis and PCR identification of the F gene.

47. We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

48. Catechol 2,3-dioxygenase(C23O) gene was amplified with the specific primer and the template from plasmid in the strain ZL5 degrading phenanthrene and pyrene.

49. Results The recombinant plasmid with mutant gene was constructed correctly.

50. Lysosome and cellular plasmid are activated to restrain activity of heavy metals.

51. Objective To investigate the amelogenin expression of recombinant plasmid PcDNA 3 - AMG in COS - 1 cell line.

52. Conclusion: pED GCSF is a high eukaryotic expression plasmid in mammalian cell.

53. With common molecular biology technique, we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid.

54. Precomputed comparisons can be selected from a database covering all the completed bacterial chromosome and plasmid sequences in the Genome Reviews database.

55. Objective : To find a rapid method of screening positive recombinant plasmid.

56. The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.

57. The vitro methods include autoagglutination test, calcium dependency test, pyrazinamidase test, salicin fermentation, esculin hydrolysis, Congo red colony test and virulence plasmid.

58. The recombinant CYP 3 A 4 plasmid was successfully cloned and effectively expressed in Rosetta ( DE 3 ) 2 pLysS.

59. ObjectiveTo study the effect of eukaryotic express plasmid of augmenter of liver regeneration (ALR) on acute hepatic failure of rats.

60. Once a plasmid is snipped open, a foreign piece of DNA, cut by the same enzyme scissors, can be taped, end to end, into the plasmid using another enzyme, DNA ligase.

61. Results The protein expressed by the plasmid which had a correct reading frame, had no transcriptional activation and could interact with a known coactivator PNRC.

62. This cut DNA is then mixed with the modified plasmid DNA, the two DNA strands join at their sticky ends and are sealed together by the enzyme, DNA ligase, to form a recombinant plasmid.

63. Methods We construct the rat antisense TIMP 1 recombinant plasmid which can be expressed in eucaryotic cells by RT Nest PCR and the technique of recombinant DNA.

64. Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor.

65. Objective To study primarily the safety of transfering recombinant human proinsulin gene plasmid(RHPGP)into diabetic rats induced by streptozotocin.

66. Objective : To survey plasmid profiles ( PP ) of Neisseria gonorrhoeae in Nanchang.

67. Objective To study the penicillin resistance in Neisseria gonorrhoeae isolates and genotypes of plasmid in penicillinase-producing N. gonorrhoeae isolates (PPNG) from Nanjing.

68. The factors influencing survival rate of LAB and plasmid stability during lyophilization were systematically studied.

69. Plasmid DNA profiles analysis offer scientific reference to molecular epidemiology investigation of avian colibacillosis .

70. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was served as the internal reference,(http:///plasmid.html) and RNA transcribed in vitro and positive plasmid were served as the standards in this experiment.

71. RAPD analysis had high distinguish ability and could provide more direct, rich and precise genetic information than plasmid profile analysis.

72. Finally a recombinant plasmid named pcNcSRS2, which was identified by PCR, restriction enzyme analysis and sequencing, was obtained.

73. Secreted proinsulin amounted to about 2 mg per liter was found in the culture medium of yeast strain 63 transformed by plasmid YTI-15.

74. Methods Isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method.

75. Aim a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

76. The result indicated, fed-batch culture could lead to the increase of thallus but without the increase of plasmid concentration.

77. ConclusionSuccessful HisTag-LRPAP1 construct encoding the fusion protein of the plasmid vector, the future can be used for eukaryotic expression of this fusion protein.

78. As a carrier system for gene therapy in animal model, VEGF-NP is much better than naked DNA plasmid. The results demonstrate great possibility of using NP carrier in human gene therapy.

79. The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.

80. Objective To control the quality of mouse nerve growth factor ( NGF ) DNA plasmid for gene therapy.

81. The relationship between penicillin resistance and R plasmid was discussed.

82. Objective To construct the plasmid expressing BDV p 40 gene.

83. The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.

84. Insert and ligate any DNA fragment into a vector such as plasmid or viral vector, to form a recombinant DNA.

85. Conclusions The recombinant plasmid constructed is useful for establishing a transgenic mouse.

86. Conclusion The recombinant plasmid that expressed the anti-human bladder carcinoma VH-PE38 immunotoxin is successfully obtained.

87. Then the recombinant plasmid was transfected into NK-92 cells with Hpofectamine encapsuled.

88. AIM: To construct plasmid vector of RNA interference specific for matrix metalloproteinases (MMP)-9 and observe the silencing effect on gene expression of MMP-9 in macrophage of mice.

89. By restriction enzyme identification, PCR and sequence analysis, the recombinant plasmid of the HA1 was successfully constructed.

90. Purified ATF - lys 10 specifically bound to uPAR - expressing cells and formed protein - DNA complexes with plasmid pGL 3 - control.

91. The screening of human tissue plasminogen activator recombinant plasmid showed that the two methods can recognize the positive colones quickly and correctly.

92. Objective To survey the antimicrobial resistance and plasmid profiles of Neisseria gonorrhoeae isolated from a hospital.

93. Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.

94. It was therefore preparation for constructing an expression plasmid vector to express exogenous genes.