1) Caffeine, phenobarbital, aminopyrine and phenacetin in antipyretic analgesic were determined using two-point internal standard method.

2) Methods: BP network spectrophotometry was selected to determine aminopyrine and phenacetin in Qutongpian.

3) Objective:To explore new methods for the determination of aminopyrine and caffeine in Naoningpian.

4) A typical drug aminopyrine, in addition to levamisole, semi-synthetic penicillin may also cause.

5) The analgesic effect of CP in mice lasted longer than Dolantin and Aminopyrine.

6) Results:The drop of body temperature in rats induced by aminopyrine was counteracted and the PKA and PKC activities increased significantly after oral administration of Guizhi Tang.