(1) Conclusion Combining laser microdissection and nested RT-PCR can monitor gene expression at a single cell level in vivo.

(2) Objective: Our aim was to evaluate the microdissection in molecular biology study of endometriosis.

(3) The principle and methods of plant microdissection and microcloning are introduced in detail, and their applications in the field of botany are summarized.

(4) Laser capture microdissection(LCM) is a novel technique developed in the recent years.

(5) Meanwhile, application of microdissection can reduce interference from the admixed non-neoplastic cell, and enhance accuracy of the assay. A total number of 95 condyloma samples were examined.

(6) Methods:Microdissection and measurement were performed on lumbosacral nerve roots of 12 adults fixed and 4 fresh children cadavers.

(7) Method The microdissection and relative data measurement were performed in the insula region of 12 adult cadaveric heads (24 cerebral hemispheres).

(8) The researchers used microdissection techniques to isolate tiny milk ducts in each sample.

(9) METHODS: RGC were obtained by microdissection and digestion, and cultured in monolayer.

(10) Methods: Microdissection were used to obtain the number, origins and anastomosis sites of the ramus communicans as well as the related data of the spinal accessory nerve .

(11) Methods Skin microdissection of pudendal regions was performed on 11 cadavers(22 sides).

(12) This study was designed to apply manual microdissection to isolate ciliated cells from guinea pig's nasal epithelium and offer pure cells to further research.

(13) Chromosome microdissection and microcloning technology was first used in animal and human. With the development of PCR, they was applied in plant genetic and evolutional researches widely.

(14) Material and Methods:From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.

(15) Methods IPE and RPE cells were isolated by enzymatic digestion method and enzyme-assisted microdissection method respectively.

(16) AIM To establish a new protocol for the determination of viral intranuclear inclusions with microdissection technique.

(17) RESULTS: Result of hematoxylin-eosin stain confirmed that the cell layer by manual microdissection was ciliated cells and basal cell layer, which were thin without submucous layer.

(18) Importantly, novel cell surface markers were identified on human bulge cells using precise laser capture microdissection and microarray analyses.

(19) Adhesion of dermal papillae, cell emigration, workload and frequency of contamination were compared with that of microdissection and microdissection with digestive treatment.