1. The substrate is a total cDNA library.

2. Recombinant cDNA were analyzed by differential screening.

3. Partial cDNA clones are shown by dotted lines at the 5' end; translation termination codons are indicated by an asterisk.

4. The cDNA clones were sequenced from both ends using standard forward and reverse primers.

5. This cDNA fragment was used to screen several murine cDNA libraries.

6. A full length Xenopus nucleolin cDNA was constructed from overlapping sequences recovered from an ovary cDNA library.

7. Numbers in brackets indicate positions in the Oct-11a cDNA sequence in Figure 1.

8. Clones were found in a mouse thymus cDNA library, however.

9. Multiple cDNA clones were identified by screening the U251 library with a bacterial lysate absorbed polyclonal antiserum against human tenascin.

10. A U251 cDNA library was screened with bacterial lysate absorbed polyclonal antiserum against human tenascin.

11. A cDNA fragment was isolated and cloned from the total RNA of calli of Onobrychis tanaitica containing condensed tannin using RT-PCR technique.

12. Generate sets of full - length cDNA clones and sequences that represent human genes and model organisms.

13. Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI.

14. Objective : To clone and sequence cDNA of human lymphotoxin ( LT ) .

15. Objective:To clone and sequence a cDNA encoding fibrinolysin metalloproteinase from the venom of Agkistrodon acutus from Guangxi.

16. Results of homology analysis revealed that the TBb cDNA sequence shared 90% homology with the major allergenic storage protein and legumin-like protein of common buckwheat.

17. A cDNA library could contain all cDNAs from a single tissue, for example, and could be screened for the ability to hybridize to a fragment of known DNA.

18. Objective : To clone the hTPO cDNA and study its expression level in COS? 7 cells.

19. A synthesis cDNA - bim ? ? 6 encodes 28 reisdues with BH 3 core sequence was built.

20. Conclusion Cationic liposome-mediated GDNF cDNA in vivo transfer can promote regeneration of corticospinal tract and facilitate repair of the cytoskeleton in the injured neurons.

21. When GAM cDNA was transfected into COS cells, it appears as an 24 kDa expressing band.

22. The 3' untranslated trailer sequence is 937 nucleotides long and no poly A tail is present indicating internal priming during cDNA synthesis.

23. The isolation of one exon is theoretically sufficient to screen cosmid and cDNA libraries for further analysis of the corresponding gene.

24. The existence of the mini-exon 3 and the positions of the adjacent intron splice points were verified by cDNA sequencing.

25. Nucleotide and predicted amino acid sequence of the Oct-11 cDNA.

26. In our hands,[] these precipitations result in progressively diminished yields of potential template cDNA for amplification.

27. These variations may be a result of slight variations in the amount of template cDNA.

28. Several deletion mutants were prepared by exploiting singular restriction sites in the cDNA to enable progressive sequencing.

29. Only one strand of the linkers ligated to the cDNA and the single strand gap was repaired with Taq polymerase prior to amplification.

30. The nucleotide and deduced amino acid sequence of the human ribosomal protein L12 cDNA.

31. The full length CMO cDNA was cloned from Salicornia europaea by RT - PCR and RLM - RACE.

32. Methods Aggrecan IGD cDNA was amplified by RT - PCR and inserted into pUCm - T vector .

33. Conclusion Co-transfer of TH and AADC cdna into the striatum is a valuable strategy to gene therapy for Parkinson's disease.

34. Results Human IL - 24 cDNA was successfully integrated into Hep - 2 cells and overexpressed.

35. The differential expression of Chinese kale during regeneration has been analyzed by cDNA - AFLP and 2 - D electrophoresis.

36. Complementary DNA (cDNA) sequence of Japanese sea bass (Lateolabrax japonicus) prion protein (PrP) encoding gene was cloned and characterized through RT-PCR approach.

37. Full - length cDNA pool is the most important step in construction of cDNA library.

38. The GenBank accession number is AF 265294. The WVDE cDNA is 1, 746 bp long.

39. Cloning of human brevican cDNA and the expression of its mRNA in human Glioma.

40. Biological information analysis showed that SSC1 had over 76% comparability to cDNA gene that encoded alcohol dehydrogenase (ADH) of Cummis melo, Prunus mume.

40. try its best to collect and make good sentences.

41. A large full - length gene bank has been contructured through large - scale cDNA cloning and sequencing.

42. The homologue of monkey ESc - 615 was cloned in rat by screening rat epididymis cDNA library.

43. The cDNA of BmK M 1 showed 87.2 % homology with that of the African scorpion Lqh α IT.

44. Methods In situ hybridization (ISH) of frozen sections of 15 human breast cancer using digoxigenin labeled estrogen receptor (ER) cDNA probe to identify the expression of ER mRNA have been done.

45. IL - 10 - cDNA fragment was obtained through cleaving pUC - T - IL - 10 cDNA by reconstriction enzymes.

46. The cDNA was subcloned into prokaryotic expression vector pET 3 d and overexpressed in E . coli BL 21 ( DE 3 ).

47. Phylogenetic trees show that there are 64 MADS - box genes encoding different proteins, including 46 cDNA sequences.

48. In order to construct the cDNA library from Antarctic ice alga Chlamydomonas sp.

49. Aim a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.

50. Results The gloverin cDNA sequence designed artificially was expressed in COS 7 cells.

51. A high throughput in silico data analysis system for cDNA library, CLASP, was constructed by bioinformatic approaches.

52. This paper focused on two aspects: the physiological effects of cold stress on the Euonymus japonicus 'Zhuzi'; the isolation of cold-induced cDNA gene.

53. E . coli DH 5α was transformed by pUC 18 recombined with PRV CP gene cDNA.

54. Objective To construct a cDNA expression library of Periplaneta Americana nymph.

55. Objective:To study the effect of dioscin to breast cancer cytogene expression by cDNA microarray technique, and to explore target genes of drug action initially.

56. Objective To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.

57. Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.

58. CONCLUSIONS: cDNA array is effective in screening candidate genes related to EOC.